Abraham Aviv, MD (Principal Investigator)
Professor and Director
The Center of Human Development and Aging
Rutgers, The State University of New Jersey
New Jersey Medical School
http://njms.rutgers.edu/departments/pediatrics/hypertension.cfm
This project focuses on comparative assessment of reliability and precision of different telomere length measurement methods. It comprises two arms: the first arm focuses on comparisons of telomere length measurements by Southern blotting and quantitative PCR (qPCR) methods in longitudinal settings. The second arm of the study incorporates telomere length measurements within the framework of a Telomere Research Network (TRN) established by the NIH.
TEAM MEMBERS
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Tsung Po-Lai, PhD,Laboratory Director |
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Shahinaz Gadalla, MD, PhD,National Cancer Institute |
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Hormuzd A. Katki, PhD,National Cancer Institute |
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Sharon A. Savage, MD,National Cancer Institute |
CURRENT LAB PROTOCOLS
Southern blot analysis of terminal restriction fragment (See Kimura M, Stone RC, Hunt SC, Skurnick J, Lu X, Cao X, Harley CB, Aviv A. Measurement of telomere length by the Southern blot analysis of terminal restriction fragment lengths. Nat Protoc. 2010 Sep;5(9):1596-1607. doi: 10.1038/nprot.2010.124. Epub 2010 Sep 2.)
KEY PUBLICATIONS
“Kimura M et al. Nat Protoc, 2010. 5(9): 1596-1607, doi: 10.1038/nprot.2010.124.”
“Daniali L et al. Nat Commun, 2013. 4: 1597, doi: 10.1038/ncomms2602.”
“Levy D et al. Proc Natl Acad Sci USA, 2010. 107(20): 9293-9298, doi: 10.1073/pnas.0911494107.”
“Wang Y et al. cells, 2018. 7(11) pii: E183. doi: 10.3390/cells7110183.”
“Dagnall C et al. PLoS One. 2017. 12 (9)”
“Gadalla SM et al. Mol Genet Genomic Med. 2016 Mar 20;4(4):475-9”
https://www.cancer.gov/news-events/press-releases/2015/donor-telomere-length-saa